Faejora Antibacterial and antifungal activity was expressed in terms of activity index AI. These plates were developed in an organic solvent mixture of benzene, acetic acid, and water The filtrate was extracted in ethyl acetate and washed with distilled water to neutrality. Geeta Singh and Padma Kumar. Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta An international multicenter study of antimicrobial consumption and resistance in Staphylococcus aureus isolates from 15 hospitals in 14 countries.
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E-mail: moc. This article has been cited by other articles in PMC. Abstract This study aims to phytochemical and antimicrobial study of Euphorbia hirta Euphorbiaceae.
Materials and Methods: Antimicrobial activity of flavonoids free and bound of Euphorbia hirta L. Total activity TA of extracts against each sensitive pathogen was also evaluated. Results: Out of fungi; A. Bound flavonoids extract of root showed best activity against C. TA of free flavonoid extract of root was found to be the same for P. Two flavonoids quercetin and kaempferol were identified in the bound flavonoids of stem extract which showed activity against all the microorganisms.
Conclusion: Results of the present investigation indicate that E. Antibiotic resistance has become a global concern. There is a continuous and urgent need to discover new antimicrobial compounds with diverse chemical structures and novel mechanisms of action for new and reemerging infectious diseases. In Nigeria, extracts or exudates of the plant are used as ear drops and in the treatment of boils, sore, and promoting wound healing. Antibacterial activity and toxicological potentials of crude ethanolic extracts of E.
Such studies could only indicate their antimicrobial potential, but are not helpful in establishing them as an antibiotic. The aim of the study was to investigate the antimicrobial activity of extracts of E.
The present investigation evaluates the antibacterial and antifungal effects of free and bound flavonoids of E. The study was carried out along with standard drugs terbinafine and itraconazol for fungus and streptomycin for bacteria. The sample specimen with No. Extraction procedure Plant parts were separately shade dried and finely powdered using a mixer. Free and bound flavonoids from root, stem leaf, and fruits of E. Each filtrate was reextracted successively with petroleum ether fraction I , ethyl ether fraction II , and ethyl acetate fraction III using separating funnel.
Petroleum ether fractions were discarded as being rich in fatty substances, whereas ethyl ether and ethyl acetate fractions were analyzed for free and bound flavonoids, respectively. The filtrate was extracted in ethyl acetate and washed with distilled water to neutrality. Ethyl ether free flavonoid and ethyl acetate fractions bound flavonoids thus obtained were dried in vaccuo and weighed.
Identification Selected extract bound flavonoid of stem which showed activity against all microorganisms tested was dissolved in ethyl acetate and applied on silica gel coated 0. These plates were developed in an organic solvent mixture of benzene, acetic acid, and water , air dried and visualized under ultraviolet UV light. Three spots retention factor Rf 0. A few other solvent system n-butanol:acetic acid: water, ; n-butanol: Water ; n-butanol:acetic acid:water, were used, but in the present investigation the solvent system of benzene, acetic acid, and water gave excellent results.
ANTIMICROBIAL ACTIVITY OF EUPHORBIA HIRTA PDF
E-mail: moc. This article has been cited by other articles in PMC. Abstract This study aims to phytochemical and antimicrobial study of Euphorbia hirta Euphorbiaceae. Materials and Methods: Antimicrobial activity of flavonoids free and bound of Euphorbia hirta L. Total activity TA of extracts against each sensitive pathogen was also evaluated. Results: Out of fungi; A. Bound flavonoids extract of root showed best activity against C.
Sunil Kumar, E-mail: moc. This article has been cited by other articles in PMC. Abstract The oldest remedies known to mankind are herbal medicines. India is known worldwide for its Ayurvedic treatment.
Full size table Among the 6. There was no significant reduction of motile mff numbers in the concentrations of R. Immobilisation of mff was observed in the The reduction of motile mff numbers occurred gradually in the crude, chloroform and aqueous extracts of E. No significant difference was found between the effect of the aqueous extract and that of the chloroform or the crude extracts. In the case of the R. In the R.